Quantitative PCR: the determination of template copy numbers by temperature gradient gel electrophoresis (TGGE)

Quantitative PCR: the determination of template copy numbers by temperature gradient gel electrophoresis (TGGE).

Quantitative PCR: the determination of template copy numbers by temperature gradient gel electrophoresis (TGGE) Low copy numbers of DNA or RNA sequences can be quantified easily by combining PCR (Polymerase Chain Reaction) and TGGE (Temperature Gradient Gel Electrophoresis). This combination allows to overcome the problems of quantitive PCR caused by plateau effects, uneven priming or, variable...

Characterization of wine yeasts by temperature gradient gel electrophoresis (TGGE).

18S rDNA from 74 wine yeast strains was amplified by PCR using specific primers, and the products analyzed by temperature gradient gel electrophoresis (TGGE). TGGE is a useful method in screening the genotypes of the wine yeasts. Intraspecific differentiation was achieved on the basis of TGGE in some cases, whereas in others identical bands for strains classified as separate species were obtain...

TGGE-STAR: primer design for melting analysis using PCR gradient gel electrophoresis.

Genotyping BoLA-DRB3 alleles in Brazilian Dairy Gir cattle (Bos indicus) by temperature-gradient gel electrophoresis (TGGE) and direct sequencing.

BoLA-DRB3 is a gene of the major histocompatibility complex (MHC) in cattle. The product of the BoLA-DRB3 gene is a beta chain of an MHC class II molecule, a glycoprotein expressed on the surface of antigen-presenting cells (APCs). Responses of CD4+ T lymphocytes to peptides are dependent on the presentation of peptide ligands bound to class II molecules on APCs. Genotyping of the BoLA-DRB3 gen...

Determination of transgene copy number and expression level using denaturing gradient gel electrophoresis.

Transgenic mice and cell lines are frequently developed to study human disease. Accurate determination of transgene copy number and levels of mRNA are necessary to understand the phenotypic changes observed in these models. Currently, transgene copy number and expression are estimated by Southern blot analysis of genomic DNA and Northern blot analysis of mRNA. We report a novel PCR-based method...